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人4-羟基苯丙酮酸双加氧酶(HPD)检测试剂盒(酶联免疫吸附试验法,宽范围)
Wide-range ELISA Kit for Human 4-Hydroxyphenylpyruvate Dioxygenase (HPD)
别名: PPD; 4-HPPD; 4HPPD; GLOD3; Glyoxalase Domain Containing 3; 4-hydroxyphenylpyruvic acid oxidase 品牌 :USCN/优尔生-A 商品货号 : UWEE251Hu 存储条件 : 4℃
规格 类别 价格 货期
96T A 1496.25 3天
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The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Wide-range 4-Hydroxyphenylpyruvate Dioxygenase (HPD). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Wide-range 4-Hydroxyphenylpyruvate Dioxygenase (HPD). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Wide-range 4-Hydroxyphenylpyruvate Dioxygenase (HPD), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Wide-range 4-Hydroxyphenylpyruvate Dioxygenase (HPD) in the samples is then determined by comparing the O.D. of the samples to the standard curve.https://www.uscnk.cn/uscn/Wide-range-ELISA-Kit-for-4-Hydroxyphenylpyruvate-Dioxygenase-(HPD)-WEE251Hu.htm