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人Ki-67蛋白(Ki-67)检测试剂盒(酶联免疫吸附试验法,宽范围)
Wide-range ELISA Kit for Human Ki-67 Protein (Ki-67)
别名: MKI67; KIA; Ki67P; Antigen Identified By Monoclonal Antibody Ki-67 品牌 :USCN/优尔生-A 商品货号 : UWEC047Hu 存储条件 : 4℃
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96T A 1496.25 3天
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The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Wide-range Ki-67 Protein (Ki-67). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Wide-range Ki-67 Protein (Ki-67). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Wide-range Ki-67 Protein (Ki-67), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Wide-range Ki-67 Protein (Ki-67) in the samples is then determined by comparing the O.D. of the samples to the standard curve.https://www.uscnk.cn/uscn/Wide-range-ELISA-Kit-for-Ki-67-Protein-(Ki67P)-WEC047Hu.htm