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人二氢二醇脱氢酶2(DDH2)检测试剂盒(酶联免疫吸附试验法)
ELISA Kit for Human Dihydrodiol Dehydrogenase 2 (DDH2)
别名: AKR1C2; DD; BABP; DD2; HAKRD; MCDR2; Aldo-Keto Reductase Family 1,Member C2; Bile Acid Binding Protein; 3-Alpha Hydroxysteroid Dehydrogenase Type III 品牌 :USCN/优尔生-A 商品货号 : USEQ200Hu 存储条件 : 4℃
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96T A 1732.50 3天
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The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Dihydrodiol Dehydrogenase 2 (DDH2). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Dihydrodiol Dehydrogenase 2 (DDH2). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Dihydrodiol Dehydrogenase 2 (DDH2), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Dihydrodiol Dehydrogenase 2 (DDH2) in the samples is then determined by comparing the O.D. of the samples to the standard curve.https://www.uscnk.cn/uscn/ELISA-Kit-for-Dihydrodiol-Dehydrogenase-2-DDH2-18029.htm