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人Ⅰ类MHC多肽相关序列A(MICA)检测试剂盒(酶联免疫吸附试验法)
ELISA Kit for Human MHC Class I Polypeptide Related Sequence A (MICA)
别名: PERB11.1 品牌 :USCN/优尔生-A 商品货号 : USEP871Hu 存储条件 : 4℃
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96T A 1732.50 3天
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The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to MHC Class I Polypeptide Related Sequence A (MICA). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to MHC Class I Polypeptide Related Sequence A (MICA). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain MHC Class I Polypeptide Related Sequence A (MICA), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of MHC Class I Polypeptide Related Sequence A (MICA) in the samples is then determined by comparing the O.D. of the samples to the standard curve.https://www.uscnk.cn/uscn/ELISA-Kit-for-MHC-Class-I-Polypeptide-Related-Sequence-A-(MICA)-SEP871Hu.htm