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小鼠氢/钾离子交换ATP酶α肽(ATP4a)检测试剂盒(酶联免疫吸附试验法)
ELISA Kit for mouse ATPase, H+/K+ Exchanging Alpha Polypeptide (ATP4a)
别名: Gastric H,K-ATPase Alpha Subunit; H(+)-K(+)-ATPase Alpha Subunit; Proton Pump; Potassium-transporting ATPase alpha chain 1 品牌 :USCN/优尔生-A 商品货号 : USEG383Mu 存储条件 : 4℃
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96T A 1620.00 3天
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The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to ATPase, H+/K+ Exchanging Alpha Polypeptide (ATP4a). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to ATPase, H+/K+ Exchanging Alpha Polypeptide (ATP4a). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain ATPase, H+/K+ Exchanging Alpha Polypeptide (ATP4a), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of ATPase, H+/K+ Exchanging Alpha Polypeptide (ATP4a) in the samples is then determined by comparing the O.D. of the samples to the standard curve.https://www.uscnk.cn/uscn/ELISA-Kit-for-ATPase--H--K--Exchanging--Alpha-Polypeptide-(ATP4a)-7066.htm