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小鼠脊椎蛋白1(SPON1)检测试剂盒(酶联免疫吸附试验法)
ELISA Kit for mouse Spondin 1 (SPON1)
别名: VSGP; F-spondin; F-spondin,Extracellular Matrix Protein; Vascular smooth muscle cell growth-promoting factor 品牌 :USCN/优尔生-A 商品货号 : USEF397Mu 存储条件 : 4℃
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96T A 1620.00 3天
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The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Spondin 1 (SPON1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Spondin 1 (SPON1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Spondin 1 (SPON1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Spondin 1 (SPON1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.https://www.uscnk.cn/uscn/ELISA-Kit-for-Spondin-1-(SPON1)-SEF397Mu.htm