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人高迁移率族框蛋白4(HMGB4)检测试剂盒(酶联免疫吸附试验法)
ELISA Kit for Human High Mobility Group Box Protein 4 (HMGB4)
别名: 品牌 :USCN/优尔生-A 商品货号 : USED772Hu 存储条件 : 4℃
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96T A 1575.00 3天
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The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to High Mobility Group Box Protein 4 (HMGB4). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to High Mobility Group Box Protein 4 (HMGB4). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain High Mobility Group Box Protein 4 (HMGB4), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of High Mobility Group Box Protein 4 (HMGB4) in the samples is then determined by comparing the O.D. of the samples to the standard curve.https://www.uscnk.cn/uscn/ELISA-Kit-for-High-Mobility-Group-Box-Protein-4-(HMGB4)-SED772Hu.htm