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大鼠3-羟基3-甲基戊二酰辅酶A还原酶(HMGCR)检测试剂盒(酶联免疫吸附试验法)
ELISA Kit for Rat 3-Hydroxy-3-Methylglutaryl Coenzyme A Reductase (HMGCR)
别名: HMG-CoA Reductase; Hydroxymethylglutaryl-CoA Reductase 品牌 :USCN/优尔生-A 商品货号 : USED686Ra 存储条件 : 4℃
规格 类别 价格 货期
96T A 1710.00 3天
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The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to 3-Hydroxy-3-Methylglutaryl Coenzyme A Reductase (HMGCR). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to 3-Hydroxy-3-Methylglutaryl Coenzyme A Reductase (HMGCR). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain 3-Hydroxy-3-Methylglutaryl Coenzyme A Reductase (HMGCR), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of 3-Hydroxy-3-Methylglutaryl Coenzyme A Reductase (HMGCR) in the samples is then determined by comparing the O.D. of the samples to the standard curve.https://www.uscnk.cn/uscn/ELISA-Kit-for-3-Hydroxy-3-Methylglutaryl-Coenzyme-A-Reductase-(HMGCR)-7052.htm