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人S100钙结合蛋白P(S100P)检测试剂盒(酶联免疫吸附试验法)
ELISA Kit for Human S100 Calcium Binding Protein P (S100P)
别名: S100-P; MIG9; S100-E; Migration-inducing gene 9 protein 品牌 :USCN/优尔生-A 商品货号 : USEC054Hu 存储条件 : 4℃
规格 类别 价格 货期
96T A 1260.00 3天
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The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to S100 Calcium Binding Protein P (S100P). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to S100 Calcium Binding Protein P (S100P). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain S100 Calcium Binding Protein P (S100P), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of S100 Calcium Binding Protein P (S100P) in the samples is then determined by comparing the O.D. of the samples to the standard curve.https://www.uscnk.cn/uscn/ELISA-Kit-for-Human-S100-Calcium-Binding-Protein-P-S100P-3530.htm