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人轴突生长诱向因子4(Ntn4)检测试剂盒(酶联免疫吸附试验法)
ELISA Kit for Human Netrin 4 (Ntn4)
别名: Beta-Netrin; Hepar-derived netrin-like protein 品牌 :USCN/优尔生-A 商品货号 : USEB835Hu 存储条件 : 4℃
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96T A 1496.25 3天
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The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Netrin 4 (Ntn4). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Netrin 4 (Ntn4). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Netrin 4 (Ntn4), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Netrin 4 (Ntn4) in the samples is then determined by comparing the O.D. of the samples to the standard curve.https://www.uscnk.cn/uscn/ELISA-Kit-for-Human-Netrin-4-Ntn4-1980.htm