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大鼠表面活性物质关联蛋白B(SP-B)检测试剂盒(酶联免疫吸附试验法)
ELISA Kit for Rat Surfactant Protein B (SP-B)
别名: SFTPB; PSPB; SFTB3; SFTP3; SPB; Surfactant Associated Protein B; Pulmonary Surfactant Protein B; 18 kDa pulmonary-surfactant protein; 6 kDa protein 品牌 :USCN/优尔生-A 商品货号 : USEB622Ra 存储条件 : 4℃
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96T A 1624.50 3天
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The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Surfactant Protein B (SP-B). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Surfactant Protein B (SP-B). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Surfactant Protein B (SP-B), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Surfactant Protein B (SP-B) in the samples is then determined by comparing the O.D. of the samples to the standard curve.https://www.uscnk.cn/uscn/ELISA-Kit-for-Rat-Pulmonary-Surfatcant-Associated-Protein-B(SP-B)-4558.htm