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大鼠花生四烯酸-5-脂加氧酶(5-LO)检测试剂盒(酶联免疫吸附试验法)
ELISA Kit for Rat 5-Lipoxygenase (5-LO)
别名： LOX5; 5-LOX; ALOX-5; ALOX5; 5LPG; LOG5; Lipoxygenase 5
品牌 ：USCN/优尔生-A
商品货号 ： USEB355Ra
存储条件 ： 4℃
| 规格 | 类别 | 价格 | 货期 | |
| 96T | A | 1624.50 | 3天 |
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产品特点：
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to 5-Lipoxygenase (5-LO). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to 5-Lipoxygenase (5-LO). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain 5-Lipoxygenase (5-LO), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of 5-Lipoxygenase (5-LO) in the samples is then determined by comparing the O.D. of the samples to the standard curve.https://www.uscnk.cn/uscn/ELISA-Kit-for-Rat-Lipoxygenase-5-LOX5-3383.htm
