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大鼠凋亡蛋白酶激活因子1(APAF1)检测试剂盒(酶联免疫吸附试验法)
ELISA Kit for Rat Apoptotic Peptidase Activating Factor 1 (APAF1)
别名: CED4; Apoptotic Protease Activating Factor 品牌 :USCN/优尔生-A 商品货号 : USEB054Ra 存储条件 : 4℃
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96T A 1624.50 3天
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The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Apoptotic Peptidase Activating Factor 1 (APAF1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Apoptotic Peptidase Activating Factor 1 (APAF1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Apoptotic Peptidase Activating Factor 1 (APAF1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Apoptotic Peptidase Activating Factor 1 (APAF1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.https://www.uscnk.cn/uscn/ELISA-Kit-for-Apoptotic-Peptidase-Activating-Factor-1-(APAF1)-8828.htm