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猪免疫球蛋白E(IgE)检测试剂盒(酶联免疫吸附试验法)
ELISA Kit for Pig Immunoglobulin E (IgE)
别名: IGHE; Immunoglobulin Heavy Constant Epsilon; Ig epsilon chain C region 品牌 :USCN/优尔生-A 商品货号 : USEA545Po 存储条件 : 4℃
规格 类别 价格 货期
96T A 1701.00 3天
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The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Immunoglobulin E (IgE). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Immunoglobulin E (IgE). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Immunoglobulin E (IgE), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Immunoglobulin E (IgE) in the samples is then determined by comparing the O.D. of the samples to the standard curve.https://www.uscnk.cn/uscn/ELISA-Kit-for-Porcine-Immunoglobulin-E-IgE-1921.htm