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小鼠Ⅳ型胶原(COL4)检测试剂盒(酶联免疫吸附试验法,小样本)
Mini Samples ELISA Kit for mouse Collagen Type IV (COL4)
别名: Type-IV Collagen 品牌 :USCN/优尔生-A 商品货号 : UMEA180Mu 存储条件 : 4℃
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96T A 1750.50 3天
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The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mini Samples Collagen Type IV (COL4). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Mini Samples Collagen Type IV (COL4). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mini Samples Collagen Type IV (COL4), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mini Samples Collagen Type IV (COL4) in the samples is then determined by comparing the O.D. of the samples to the standard curve.https://www.uscnk.cn/uscn/Mini-Samples-ELISA-Kit-for-Collagen-Type-IV-(COL4)-MEA180Mu.htm